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| Registros recuperados: 124 | |
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| Rêgo,Moacyr J. B. M; Cavalacanti,Carmelita L. B; Beltrão,Eduardo I. C; Sobral,Ana Paula V. |
| Carbohydrates play a critical role in many cellular processes like disease, growth and development. In this work lectins, proteins that recognizes carbohydrate free or conjugated, were used as histochemical probes for carbohydrates localization in developing human minor salivary gland. Immunohistochemistry for traditional cytoskeleton markers (Cks 7, 8, 13, 14, 19, SMA and Vimentin) was performed and then compared whit lectin histochemistry for PNA, WGA, ConA and UEA-I, specifics for D-galactose, N-acetyl-glucosamine, glucose/mannose and L-fucose respectively. For this, specimens were obtained from tongues and lips of 15 human foetuses at 10-28 weeks of gestation. None of immune cytoskeleton markers were identified in the first stage of development... |
| Tipo: Journal article |
Palavras-chave: Lectin histochemistry; Immunohistochemistry; Developing human minor salivary glands. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200051 |
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| Riccillo,F.L.; Bracamonte,M.I.; Cónsole,G.M.; Gómez Dumm,C.L.A.. |
| Although the endocrine pancreas is the purpose of several deep investigations, morphological data referred to the effect of aging on the gland are not homogeneous. The purpose of the current work was to analyze the changes occurring in the pancreas of aged rats, with especial reference to the islet cell populations. Six young (Y), old (O) and senescent (S) male Sprague-Dawley rats were used. The pancreas tails were processed for light microscopy and studied by means of routine stains as well as by immunohistochemical identification of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide- secreting cells (Dako Envision System, DAB as chromogen). A progressive pancreatic histoarchitecture distortion was found among the aged animals. Even when the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aging; Pancreas; Islets; Immunohistochemistry; Morphometry. |
| Ano: 2004 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200001 |
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| Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da. |
| ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine visceral leishmaniasis; Structural changes; Genital pathology; Immunohistochemistry. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641 |
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| Shen,Cong-Cong; Kang,Yu-Huan; Yu,Lin; Cui,Dan-Dan; He,Yi; Yang,Jin-Liang; Gou,Lan-Tu. |
| BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in... |
| Tipo: Journal article |
Palavras-chave: Immunohistochemistry; TEX101; Seminoma; Testis; Western blotting. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075 |
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| Sá,V.K. de; Rocha,T.P.; Moreira,AL.; Soares,F.A.; Takagaki,T.; Carvalho,L.; Nicholson,A.G.; Capelozzi,V.L.. |
| We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hyaluronidases and hyaluronan synthases; Lung cancer; Pre-neoplastic lung/bronchial lesions; Immunohistochemistry; Morphometry; Prognosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101039 |
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| Estima-Silva,P.; Riet-Correa,F.; Coelho,A.C.B.; Echenique,J.V.Z.; Marcolongo-Pereira,C.; Lima,M.; Diel,D.G.; Schild,A.L.. |
| RESUMO Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Abortion; Equine herpesvirus-1; Immunohistochemistry; PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401421 |
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| Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin. |
| The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members... |
| Tipo: Text |
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf |
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| Oliveira,David Moraes de; Silveira,Márcia Maria Ferreira da; Andrade,Emanuel Sávio de Souza; Sobral,Ana Paula Veras; Martins-Filho,Paulo Ricardo Saquete; Santos,Thiago de Santana; Oliveira,Patrícia Leimig Amorim de; Peixoto,Aline Carvalho; Santos,Jadson Alípio Santana de Souza; Piva,Marta Rabello. |
| This study investigated the immunodetection of PTCH in epithelial components of dental follicles associated with impacted third molars without radiographic signs of pathosis. One hundred and five specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferation was determined by using immunohistochemical labeling. Statistical analysis was performed using Fisher exact test and a level of significance of 5%. Of the 105 dental follicles collected, 3 were PTCH-positive. The specimens with squamous metaplasia and epithelial hyperplasia had higher rates of positivity for PTCH, as well as those with active remnants of odontogenic... |
| Tipo: Journal article |
Palavras-chave: Immunohistochemistry; Dental follicle; Odontogenic epithelial; PTCH. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100018 |
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| Namm,Aimar; Arend,Andres; Aunapuu,Marina. |
| The role of bone morphogenetic proteins (BMP-s) in the development of the nervous system has been widely studied on avian and rodent embryos. Human embryos have rarely been available for detection of BMP expression. In this study 39 human embryos of Carnegie stages (CS) 10-20 were investigated. The embryos were fixed in paraformaldehyde, embedded in paraffin and sectioned serially in transverse direction. BMP-2 and BMP-4 protein expression in the developing neural tube and the caudal spinal cord was determined by immunohistochemistry. Our data show that BMP-s tend to be more expressed in the neural tube in earlier stages; in particular, BMP-4 staining was found to be higher at CS10 compared to CS20. More detailed analysis was performed on embryos of... |
| Tipo: Journal article |
Palavras-chave: Neural tube; Human embryo; BMP-2; BMP-4; Immunohistochemistry. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200018 |
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| Santos,G.C. dos; Parra,E.R.; Stegun,F.W.; Cirqueira,C.S.; Capelozzi,V.L.. |
| Idiopathic interstitial pneumonias include complex diseases that have a strong interaction between genetic makeup and environmental factors. However, in many cases, no infectious agent can be demonstrated, and these clinical diseases rapidly progress to death. Theoretically, idiopathic interstitial pneumonias could be caused by the Epstein-Barr virus, cytomegalovirus, adenovirus, hepatitis C virus, respiratory syncytial virus, and herpesvirus, which may be present in such small amounts or such configuration that routine histopathological analysis or viral culture techniques cannot detect them. To test the hypothesis that immunohistochemistry provides more accurate results than the mere histological demonstration of viral inclusions, this method was applied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrosing interstitial pneumonia; Viral infections; Immunohistochemistry; Tissue microarray. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100985 |
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| López,María José; Zárate,Michael; Cantín,Mario; Zavando,Daniela; Suazo Galdames,Iván. |
| The vocal muscle is a striated muscle with important functions in the emission of laryngeal sound and physiology of the voice. Therefore the knowledge of its constitution is the basis for the prevention and management of voice disorders. We used 10 samples from the middle third of vocal muscles obtained from autopsies of 6 male and 4 female subjects aged between 36 and 71 years. The samples were analyzed with BA-F8 monoclonal antibody to slow type I fibers, and antimyosin HC monoclonal antibody and antimyosin fast clone MY-32 antibody for types IIA, IIB, IIX, and neonatal fibers. We determined the distribution of the muscle fiber types and morphometric characteristics, evaluating the differences by sex and age group. The human vocal muscle presented a... |
| Tipo: Journal article |
Palavras-chave: Voice; Vocal cords; Laryngeal muscles; Myosin Type I; Myosin Type II; Immunohistochemistry. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400014 |
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| An,Fengli; Wang,Jianlin. |
| Spermatogenesis is associated with considerable fluid secretion or absorption in the male reproductive tract. Aquaporins (AQPs) are membrane protein channels that allow the rapid movement of water through epithelium. In the present study, immunohistochemistry was utilized to localize the expression of AQP 1, AQP2 in the testis and prostate of adult bactrian camel (Camelus bactrianus). Results show that AQP1 have intense reaction in rete testis, efferent ducts, vessels, seminiferous duct and in the prostate, AQP2 was found minor expression in the rete testis, vessels and prostate, which suggesting that AQP1 may have the main role in the absorption of the large amount of testicular fluid in male camel reproductive tract. Investigations of AQPs biology in... |
| Tipo: Journal article |
Palavras-chave: Aquaporin1; Aquaporin2; Immunohistochemistry; Bactrian Camel. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400007 |
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| Rojas,Mariana; Saint-Pierre,Gustavo; Hartley,Ricardo; Vásquez,Bélgica; Conei,Daniel; del Sol,Mariano. |
| With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spatial location of the Sonic Hedgehog (Shh) morphogen in hatched fry stage. Fifteen salmon fry (Salmo salar) were used. They were anesthetized with 5 % benzocaine (BZ-20®, Veterquímica), fixed in 10 % buffered formalin, and embedded in paraffin, Shh polyclonal antibody (Santa Cruz H-160, rabbit) was used diluted at 1/100. They were subsequently rinsed in PBS-1 % Triton and incubated with anti-rabbit conjugated polymer antibody and HRP for 10-15 min. The... |
| Tipo: Journal article |
Palavras-chave: Sonic hedgehog; Salmon fry; Immunohistochemistry; Antibody antiShh. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200054 |
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| Registros recuperados: 124 | |
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